Lone Pine Koala Sanctuary is an 18-hectare (44-acre) koala sanctuary in the Brisbane suburb of Fig Tree Pocket in Queensland, Australia. Founded in 1927, it is the world's oldest and largest koala sanctuary. With over 90 years of experience, Lone Pine is home to 130 koalas and over 70 species of other native Australian animals. 

The name originates from a lone hoop pine that was planted by the Clarkson family, the first owners of the 4.6-hectare (11-acre) site.  The sanctuary opened in 1927 to provide a safe refuge to sick, injured, and orphaned koalas, at a time when they were being killed for their fur. The founder of the sanctuary, Claude Reid, recognised the need to protect this iconic species and initiated the protection of their habitat.

The sanctuary began with two koalas called Jack and Jill. Lone Pine became known internationally during World War II when Americans, including Douglas MacArthur's wife, visited the park to view the native Australian animals. Wildlife in the sanctuary includes: koalas, kangaroos, Tasmanian devils, wombats, echidnas, and various species of reptiles, as well as a platypus that arrived at the sanctuary during 2010 from Healesville Sanctuary (near Melbourne).

Visitors are allowed to hold koalas for a fee. Strict regulations ensure that each koala is not held for more than thirty minutes every day. Fees paid for souvenir photos help fund new enclosures, research projects and eucalyptus plantations.  

According to visitbrisbane.com.au